Summary: The dual interrogation of the transcriptome and proteome with single-cell resolution provides exquisite insights into immune humboldt cherry cider mechanisms in health and disease.Here, we describe a cutting-edge protocol wherein we combine Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-Seq), a technique utilizing antibody-oligonucleotide conjugates (AOCs), with fluorescence-activated cell sorting to enrich rare cell populations.Our protocol incorporates co-staining of cells with both fluorescent antibodies and AOCs simultaneously for subsequent input into the cell sorting and CITE-Seq pipeline.
For complete bengals dog collar details on the use and execution of this protocol, please refer to Mair et al.(2020).